| CONTENTS
| OVERHEADS |
GLOSSARY
| REFERENCES
| SKILLS |
MATERIALS 
10/2/97 see also SUPPLIERS
The following is a list of materials for each of the lessons. These are, for
the most part, the same materials lists as in the lessons themselves. Having
it all in one place allows you to see at a glance if you have or can get the
materials necessary BEFORE you commit to learning about or applying a new lesson.
FOR SOFTWARE see HOW WE DID IT
Many of the lessons use the "Urchin Kit" which
consists of the following:
- 0.5M potassium chloride solution (3.7grams of KCL in 100ml of distilled
water)
- sea water (or Instant Ocean, or artificial sea water)
- fertile sea urchins
- 1-5cc syringe with #25-#30 needle
- plastic or glass pasteur pipets or eyedroppers
- small tubes to store sperm (micro-centrifuge tubes work great) (1-5 milliliters)
- beakers a little smaller in diameter than the diameter of female urchins
NOTE: if possible use glassware that has never be exposed to soap
or detergent as even the smallest amount can disrupt development.
- computer to show animations
- microscopes
- Optional - chloramphenicol for long term storage of eggs (30 micrograms/ml)
in sterile sea water.
- Optional - video microscopy setup (see VIDEO MICROSCOPY)
- Overhead to show overhead transparencies
- Urchin Kit
- incubator to store developing embryos at desired temperature (see below)
- Urchin Kit
- sumi ink - an ink used in Asian countries for calligraphy. Available in
most art stores.
- Urchin Kit
- incubator to store developing embryos at desired temperature (see below)
- Urchin Kit
- incubator to store developing embryos at desired temperature (see below)
- "Toxins" - students choose, but could be household materials (Clorox, paint,
oil, etc)
- Environmental Conditions - heat, light, cold, pH, etc.
IDEAS FOR AN INCUBATOR FOR TEMPERATURE STUDIES AND CULTURING EMBRYOS
- go to a garage sale and get a used refrigerator. turn the temperature to
the WARMEST setting. This usually works out to
about 15C, perfect for a lot of urchins.
- make an "aquarium heater" incubator to be stored in your refrigerator. use
this technique if you cannot dedicate a refrigerator for the "warmer" temperature.
Simply purchase a thick Styrofoam box or cooler and put a small 25 watt aquarium
heater inside supported to be away from the walls. adjust the thermostat until
you get the desired temperature (this will take some time so don't wait until
the last moment!). The thicker the Styrofoam the smaller the aquarium heater
needs to be and the less strain on your refrigerator.
- Sea urchin sperm
- Refrigerator
- "Toxins" - students choose, but could be household materials (Clorox, paint,
oil, etc)
- Environmental Conditions - heat, light, cold, pH, etc.
- microscopes
- one depression slide with a cover slip or a regular slide with 3 cover slips
- Urchin Kit or other live/prepared material
- microscopes
- prepared slides or living material
- overhead projector
- printout of "figure" from lesson
- The Game Cards -- There is one for sexual
and one for asexual. These can be enlarged or projected
easily. Print the cards onto a transparency material to use with an overhead.
- THE "GENES" - The various PHENOTYPES
are represented by a "cast of characters". A CHARACTER
sheet is provided so you can make the many game pieces you will need.
- SCENARIOs - print these out and use as is or
adapt to help make the point you are trying to get across.
- OVERHEAD PROJECTOR to either do or demo.
- ability to make empty space in classroom
- overhead or chalkboard
- computer to show animation
- "Classic Coke" - one can per lab group (they will sample it I'm sure)
- ICE in a Styrofoam container.
- water to make dilutions with
- graduated cylinder (10 ml will work fine)
- clean test tubes (16x100 work great), 8 for each lab group
Spectrophotometer parts: (has the potential of being shared with the chemistry
classes and used to measure other reactions like enzyme kinetics or bacteria/yeast
growth)
- pkg of 5 photocells - Radioshack #276-1657, $2.29/pkg
- digital multimeter - Radioshack #22-166, $39.99 ea
instructor may want the Radioshack #22-168 at $129.99 ea, as this will interface
with a computer that can then be projected onto a television with the proper
adaptor.
- Black foam core board from an art supply store
- black tape - black 3M #235 photographers tape works best, but duct tape
will also work
- Blue filter - suggest filter pack from Edmund Scientific #A60,373 $16.75
This will give plenty of material to be cut up for class use in this lessons
and others.
- Light source - can be a microscope lamp, large flashlight, direct sunlight,
etc.
- one small package of assorted food colors for each lab group. (Larger size
bottles should be purchased for refills for future classes.)
- a light table, students working in groups of 2. (A sheet of translucent
white plastic over a sunlit window works great!)
OR
use the spectrophotometers from the SIMPLE DILUTION
lesson, students working in groups of 4-5
- set of colored filters (Edmund Scientific #A60,373 $16.75), colored cellophane,
or colored transparencies. Make sure they have several colors, so they are
forced to make a choice.
- set of 10 clean test tubes of the same size & test tube rack
- set of unknowns made up ahead of time, dilute but still showing visible
color. Too high a concentration and the students may think they have solved
the problem too easily.
- graduated cylinders (10ml) and eye droppers
- water, paper towels, etc. This lab can be messy!
- See Spectrophotometer in Simple Dilution lesson plan. Use the same BLUE
filter for measuring sperm concentration.
- hemacytometer - Fisher Scientific #02-671-10 $96
- Urchin Kit
- test tubes (to hold 10 ml)
- plastic pipets calibrated to hold 1 ml (E&K Scientific #500025 pkg/1000
~$18)
- 10 ul Wiretrol - Fisher Scientific #21-175B pkg/100 $15.25
- paper, pencils, overhead projector and overhead
- optional, timer
Look at individual topics. These ideas all interrelate and it would be hard
here to give a good list of materials needed.
S.U.E. - CONTENTS