SPERM DILUTION

Tube #

Dilution

# of sperm per small square

K-Ohms
(spectrophotometer)

sperm per ml (calculated)

1 (stock)

1:1000

     

2

1:2000

     

3

1:4000

     

4

1:8000

     

5

1:16,000

     

6

1:32,000

     

7

1:64,000

     

8

1:128,000

     

BLANK

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[sperm/ml = 4,000,000 x sperm per small square on hemacytometer]

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