BLEACH

3/31/1999

Doing a wash load with bleach requires one cup bleach [5.25% sodium hypochlorite] per wash load [16 gallons] or one part in 256.

TUBE #

DIRECTIONS:

TOTAL DILUTION

1

1 part bleach & 9 parts seawater

1 : 10

2

1 part #1 and 9 parts seawater

1 : 100

3

1 part #2 and 9 parts seawater

1 : 1,000

4

1 part #3 and 9 parts seawater

1 : 10,000

5

1 part #4 and 9 parts seawater

1 : 100,000

6

1 part #5 and 9 parts seawater

1 : 1,000,000

7

1 part #6 and 9 parts seawater

1 : 10,000,000

8

PURE SEAWATER

CONTROL

Set up test tubes with about a half an inch of each of the above solutions.

  1. Add one drop of concentrated eggs to each.
  2. Swirl and let sit for one minute.
  3. Add one drop of diluted sperm suspension and swirl briefly.
  4. Wait 5 minutes.
  5. Score eggs for fertilization. If time permits can also score for later development [Next period see if divided. Next day see if there are swimming blastula.]